CYTOLOGICAL TECHNIQUES
The processes that are implemented to observe cells and their components are said to be cytological techniques. There are 5 basic methods via which cytological specimens are studied:
- Teasing
- Smear preparation
- Squash preparation
- Whole mounting
- Microtomy
Teasing: Most of the musculature is observed by teasing with saline solution. A Safranin or Eosin stained specimen is viewed under microscope.
Smear preparation: It is a film of a fluid tissue which is dried, stained, covered with a cover glass and observed under the desired model of microscope.
smear of different samples
Squash preparation: Soft tissues such as onion root tip, testes are prepared by softly pressurizing them into soft thin layers within cover glasses.
squash of onion root tip cell
Whole mounting: The process of mounting a specimen as such without any of the above mentioned preparatory processes is said to be whole mounting.
Microtomy: It is the mechanism of producing thin, transparent sections of cells, tissues which are further subjected to temporary or permanent microscopic studies. Detailed studies on microscopic specimen can be made on cells and tissue samples which undergo a series of physical and chemical treatments. The treatments are listed as follows:
- Fixation
- Dehydration
- Clearing
- Embedding
- Sectioning
- Staining
- Mounting
- Labelling
Let us unravel each of the above mentioned treatments in the fore-coming blogs.
Until then stay tuned.
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